Recent advances in technological solutions for automated high-speed and high-resolution whole slide imaging (WSI) have set the basis for a digital revolution in microscopy. This ability to observe and analyze entire specimens rather that single microscopic fields of view is affecting the way microscopic evaluation is practiced. However, WSI outputs quite huge multiple channel (at least three color channels) images (e.g. 30-40 GB) for a single slide and managing such amount of data is a unique challenge for this new era of digital microscopy. Currently, WSI workstations are mainly used to perform virtual microscopy, the practice of converting entire glass slides into high-resolution digital slides that can be viewed and managed across networks. The aim of the proposed special issue is to present some of the cutting-edge works currently being done in Whole Slide Imaging and reveal the challenges that still lie ahead. The special issue will be a mix of invited and solicited papers. A pers...